不同耐旱基因型玉米伤流及其伤流液中碳、氮代谢相关成分含量分析

Bleeding Sap and Its Nutrient Concentrations Related to Carbon and Nitrogen Metabolism of Maize Genotypes with Different Drought-tolerance

利用田间实验,以耐旱基因型玉米"掖单13"和不耐旱基因型玉米"丹玉13"为试材,于苗期、拔节期、孕穗期和灌浆期取样,测定伤流强度和伤流液中的可溶性糖、可溶蛋白含量和游离氨基酸含量,以期从伤流液角度分析玉米耐旱机理。结果显示,生育后期玉米伤流强度小于生育前期;伤流液中的可溶性糖含量是苗期最低,拔节期、孕穗期和灌浆期其含量较高;苗期和灌浆期伤流液中的可溶蛋白和游离氨基酸含量较高,而拔节期和孕穗期其含量相对较低。耐旱基因型玉米在拔节期、孕穗期和灌浆期其伤流液中的可溶性糖含量和游离氨基酸含量显著高于不耐旱基因型玉米(p<0.05)。图4,参23。

Taking drought-tolerant maize variety Yedan-13 and drought-sensitive maize variety Danyu-13 as test materials,a field experiment was conducted to study the intensity and soluble sugar,soluble protein,and free amino acid contents in the bleeding sap at the seedling,jointing,booting,and filling stages,with an aim to understand the mechanisms of maize drought-tolerance from the viewpoint of bleeding sap.The results showed that the bleeding intensity was greater at seedling and jointing stages than at booting and filling stages.The soluble sugar content in the bleeding sap was the lowest at seedling stage but higher at jointing,booting,and filling stages,whereas the soluble protein and free amino acid contents were higher at seedling and filling stages but relatively lower at jointing and booting stages.As compared with drought-sensitive maize variety,drought-tolerant maize variety had significantly higher contents of soluble sugar and free amino acid contents in the bleeding sap at jointing,booting,and filling stages(p<0.05).