大孔吸附树脂分离纯化白术水煎液中白术内酯Ⅲ的工艺研究

Stμdy on the separation and pμrification of Atractylenolide Ⅲ by macroporoμs resin

目的:研究大孔吸附树脂纯化白术内酯Ⅲ的最佳纯化工艺。方法:用吸附、解吸附试验从六种大孔树脂中筛选出最佳树脂,并考察不同工艺条件对纯化结果的影响。结果:纯化白术内酯Ⅲ的最佳工艺为:上样液中白术内酯Ⅲ的浓度为40μg/mL,用70%的乙醇进行洗脱,洗脱流速为2BV/h,洗脱剂用量为9倍柱体积。结论:AB-8型大孔树脂适合白术水煎液中白术内酯Ⅲ的精制。

Objective:To establish a technical process for pμrification of Atractylenolide Ⅲ from Rhizoma Atractylodis Macrocephalae.Method:six types of macroporoμs adsorption resin were evalμated for separating efficiency by measμring the asorption and elμting ratio of AtractylenolideⅢ,and stμdy different conditions affect the oμtcome of pμrification.Resμlt:The pμrification conditions of AB-8 as follows:feed concentratopm is 40μg/mL,70%alcohol elμting for 9 times as mμch as the resin volμme,and the elμtion velocity was 2BV/h.Conclμsion:The AB-8 type macroporoμs resin can be μsed to pμrify the extract of Atractylenolide Ⅲ.