XE-2100全自动血细胞分析仪检测外周血造血干/祖细胞

Determination of peripheral blood stem/progenitor cells by XE-2100 fully automatic hematology analyzer

目的探讨XE-2100全自动血细胞分析仪在检测外周血造血干/祖细胞(HSC/HPC)的临床应用。方法采用XE-2100全自动血细胞分析仪上的未成熟细胞信息(IMI)通道和流式细胞术(FCM)分别对10例健康新生儿脐血、20例白血病患者及1例异体外周血干细胞移植(Allo-PBSCT)供者经动员后不同时间外周血中造血干/祖细胞动态变化进行检测。结果在脐血和PBSCT供者的外周血中,IMI检测的HSC/HPC的百分率与FCM检测的CD34+ 细胞的百分率有良好的相关性,r =0.82(P<0.01)。标本批内变异系数(CV)小于10%;对存在有幼稚细胞的白血病患者,两种方法的相关性差。供者经动员后,HPC与CD34+ 细胞同时上升,于第5天同时出现峰值。结论在同种异体外周血干细胞移植(Allo-PBSCT)时,选择健康人作供体,XE-2100全自动血细胞分析仪能简捷、快速、费用经济地检测外周血造血干/祖细胞,为临床上判断外周血干细胞最佳采集时机提供了一种新的参考方法。

Objective: To explore the clinical application of XE-2100 automatic hematology analyzer in determination of hematopoietic stem/progenitor cells in peripheral blood. Methods: 10 samples of heahthy crod blood, the peripheral blood of 20 leukemia patients and 1 allogeneic peripheral blood stem cells transplantation (Allo-PBSCT) patients were detected separately with the Immature Nyeloid Information (IMI) channel of XE-2100 automated hematology analyzer and Flow Cytometry (FCM). Results: There is high co-relative between the percentage of HSC/HPC detected by IMI and CD34+ cells detected by FCM, the sample of which were collected from umbilical cord blood and PBSCT donors, r =0.82 (P<0.01), the inside coefficient of variation (CV)<10%, while the co-relative is low between the leukemia patients with Immatured Cells. HPC and CD34+ cells in Allo-PBSCT donors remarkably increased on the 5th day of mobilization. Conclusions: XE-2100 automatic hematology analyzer provides fast, convenient, accurate and economical method to detect peripheral blood stem cells of Allo-PBSCT donors and a new way to choose the proper time collecting peripheral blood stem cells.