医院感染血浆凝固酶阴性葡萄球菌耐药性分析

Resistance of nosocomial coagulase negative staphylococci

目的 了解医院感染血浆凝固酶阴性葡萄球菌 (coagulasenegativestaphylococciCONS) β -内酰胺酶 ,粘质物产生等耐药性的情况。方法 对临床分离CONS予以鉴定 ,琼脂纸片扩散法 (K -B)检测药敏 ,Ni trocefin法检测 β -内酰胺酶 ,刚果红平板法检测粘质物。 结果 临床分离CONS对青霉素、红霉素、复方新诺明、苯唑西林、环丙沙星、克林霉素的耐药率较高 ,分别为 85 .4 %、81.0 %、79.8%、6 7.7%、5 8.2 %和5 5 .1% ,对氨苄西林 /舒巴坦、利复平、美洛培南较敏感 ,耐药率分别为 8.9%、17.1%和 2 4 .7% ,未发现有耐万古霉素的菌株。β -内酰胺酶阳性率为 72 .8% ,粘质物阳性率为 8.9%。 结论 CONS耐药性增高与其产β -内酰胺酶、粘质物有关 ,同时还会导致耐甲氧西林凝固酶阴性葡萄球菌 (methicillin -resistantcoagulasenega tive,staphylococciMRCONS)出现。

Objective:To study the resistance to antibiotics of nosocomial coagulase negative staphylococci (CONS ) and detect the β-lactamase and the slime of CONS.Methods:To identify the nosocomial CONS and study the resistance of CONS, we use Kirby-Bauer methods. To detect the β-lactamase and the slime, we use the Nitrocefin methods and the Congo Red method respectively.Results:The resistance rates of CONS to Penicillin, Erythromycin,Trimethoprim-sulfamethoxazole,Oxacillin,Ciprofloxacin and Clindamycin were 85.4%,81.0%, 79.8%,67.7%, 58.2% and 55.1% respectively, to Ampicillin/sulbactam, Rifampin and Meropenem were 8.9%,17.1% and 24.7% respectively. None of the CONS was resistant to Vancomycin. The positive rates of the β-lactamase and the slime were 72.8% and 8.9%, respectively.Conclusions:The raise of the resistance of CONS was relative to the β-lactamase, the slime and MRCONS.