流动注射化学发光测定盐酸多巴酚丁胺

Determination of Dobutamine Hydrochloride by Flow-injection Chemiluminescence Method

基于盐酸多巴酚丁胺对高锰酸钾氧化鲁米诺产生的化学发光的增强作用建立了一种测定盐酸多巴酚丁胺的新方法.在优化的实验条件下,测定盐酸多巴酚丁胺的线性范围为1 0×10-3～0 5μg/mL,检出限(3σ)为5 0×10-4μg/mL,对浓度为0 4μg/mL的盐酸多巴酚丁胺进行11次平行测定,其相对标准偏差(RSD)为3 39%.将本法用于注射液、血样以及尿样中盐酸多巴酚丁胺的测定,结果令人满意.

A new chemiluminescence (CL) method, which based on enhancement effect of dobutamine hydrochloride on the CL intensity produced by potassium permanganate oxidation on luminol is proposed for the determination of dobutamine hydrochloride. Under optimized conditions, the response of CL on concentration of dobutamine hydrochloride is linear in the range 1.0×10^(-3)-0.5 μg/mL with a detection limit (3σ) of 5.0×10^(-4) μg/mL. Relative standard deviation (RSD) is 3.39% for 0.4 μg/mL dobutamine hydrochloride (n=11). The proposed method has been successfully applied to the determination of dobutamine hydrochloride in pharmaceutical preparations, plasma and urine samples with satisfactory results.