期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

生长激素释放肽对高糖诱导下胰岛β细胞凋亡的影响 被引量：1

原文传递
导出
摘要 目的探讨生长激素释放肽(Ghrelin)对高糖诱导的胰岛β细胞凋亡的影响。方法根据不同的培养基将胰岛β细胞株NIT-1细胞划分为四组:A组(5.6mmol/L葡萄糖全程),B组(5.6mmol/L葡萄糖+10-7mol/L Ghrelin24h,5.6mmol/L葡萄糖72h),C组(5.6mmol/L葡萄糖24h,33.3mmol/L葡萄糖72h),D组(5.6mmol/L葡萄糖+10-7mol/L Ghrelin24h,33.3mmol/L葡萄糖培养72h)。流式细胞仪Annexin V/PI双染色法测定四组NIT-1细胞早期凋亡率;Hoechst33258标记细胞核并在荧光显微镜下测各组细胞总凋亡率;RT-PCR法测定Bcl-2、Bax mRNA水平。结果 (1)与A组比较,B组NIT-1细胞早期凋亡率及总凋亡率均无统计学意义(P均>0.05),而C组NIT-1细胞早期凋亡率及总凋亡率均显著增加(P均<0.01);与C组比较,D组NIT-1细胞早期凋亡率及总凋亡率均降低(分别P<0.01,P<0.05)。D组较A组细胞早期凋亡率有增加(P<0.05),而总凋亡率无统计学意义(P>0.05)。(2)与A组比较,B组NIT-1细胞Bax、Bcl-2、Bcl-2/Bax表达水平无变化,而C组Bcl-2、Bcl-2/Bax水平显著下降(P<0.05);与C组比较,D组Bcl-2、Bcl-2/Bax表达有升高趋势,但无统计学意义;各组Bax表达水平无差别。结论 Ghrelin对葡萄糖生理浓度下的胰岛β细胞凋亡无影响,但可抑制高浓度葡萄糖诱导的β细胞凋亡。Ghrelin可能不通过Bcl-2、Bax抑制高糖诱导的β细胞凋亡。
作者 马淑芳 林少达 林楚佳
机构地区 汕头大学医学院第一附属医院内分泌科
出处 《中华临床医师杂志（电子版）》 CAS 2010年第7期1064-1067,共4页 Chinese Journal of Clinicians(Electronic Edition)
基金 广东省科技计划项目(2005B30701006)
关键词 生长激素释放激素 胰岛素分泌细胞 细胞凋亡 糖尿病
分类号 R587.1 [医药卫生—内分泌]
  • 相关文献

参考文献2

二级参考文献18

  • 1Date Y, Nakazato M, Hashiguchi S, et al. Ghrelin is present in pancreatic alpha-cells of humans and rats and stimulates insulin secretion. Diabetes, 2002,51 ( 1 ) : 124-129.
  • 2Volante M, Allia E, Gugliotta P, et al. Expression of ghrelin and of the GH secretagogue receptor by pancreatic islet cells and related endocrine tumors. J Clin Endocrinol Metab, 2002,87 ( 3 ) : 1300-1308.
  • 3Prado CL, Pugh-bernard AE, Elghazi L, et al. Ghrelin cells replace insulin-producing beta cells in two mouse models of pancreas development. Proc Natl Acad Sei U S A ,2004,101 (9) : 2924-2929.
  • 4Kojima M, Hosoda H, Date Y, et al. Ghrelin is a growthhormone-releasing acylated peptide from stomach. Nature, 1999,402(6762) :656-660.
  • 5Gormsen LC, Nielsen C, Gjedsted J, et al. Effects of free fatty acids, growth hormone and growth hormone receptor blockade on serum ghrelin levels in humans. Clin Endocrinol (Oxf) ,2007,66(5):641-645.
  • 6Irako T, Akamizu T, Hosoda H, et al. Ghrelin prevents development of diabetes at adult age in streptozotocin-treated newborn rats. Diabetologia,2006,49 (6) : 1264 -1273.
  • 7Granata R, Settanni F, Biancone L, et al. Acylated and unacylated ghrelin promote proliferation and inhibit apoptosis of pancreatic beta-cells and human islets: involvement of 3', 5'-cyclic adenosine monophosphate/protein kinase A, extracellular signal-regulated kinase 1/2, and phosphatidyl inositol 3-Kinase/Akt signaling. Endocrinology, 2007,148 ( 2 ) : 512-529.
  • 8Yang M, Hu S, Wu B, et al. Ghrelin inhibits apoptosis signal-regulating kinase 1 activity via upregulating heat-shock protein 70. Biochem Biophys Res Commun, 2007,359 (2) : 373 -378.
  • 9Dezaki K, Hosoda H, Kakei M, et al. Endogenous ghrelin in pancreatic islets restricts insulin release by attenuating Ca2+ signaling in beta-cells: implication in the glycemic control in rodents. Diabetes,200d,53(12) :3142-3151.
  • 10Esler WP, Rudolph J, Claus TH, et al. Small-molecule ghrelin receptor antagonists improve glucose tolerance, suppress appetite, and promote weight loss. Endocrinology, 2007,148(11) :5175-5185.

共引文献1

同被引文献11

  • 1Dczaki K, Hosoda H, Kakei M, et al. Endogenous ghrelin in pancreatic islets restricts insulin release by attenuating Ca2+ signaling in beta-cells: implication in the glycemic control in rodents[/]. Diabetes, 2004, 53(12): 3142-3151.
  • 2McMurray JJ, Holman RR, Haffner SM, et al. Effect of valsartan on the incidence of diabetes and cardiovascular events[J]. N Engl J Med Overseas Ed, 2010, 362(16): 1477-1490.
  • 3Bosch J, Yusuf S, Gerste/a HC, et al. Effect of ramipril on the incidence of diabetes[J]. N Engl J Med Overseas Ed, 2006, 355(15): 1551-1562.
  • 4Yin GS, Lin SD, Xu DC, et al. Handle region peptide ameliorating insulin resistance but not β cell functions in male rats neonatally treated with sodium L-glutamate[J]. Int J Endocrinol, 2013.
  • 5Dawson R Jr, Wallace DR, Gabriel SM. A pharmacological analysis of food intake regulation in rats treated neonatally with monosodium L-glutamate(MSG)[J]. Pharmaeol Bioebem Behav, 1989, 32(2): 391-398.
  • 6NemeroffCB, Konkol ILl, Bissette G, et al. Analysis of the disruption in hypothalamic-pituitary regulation in rats treated neonatally with monosodium L-glutamate (MSG): evidence for the involvement of tuberoinfundibular cholinergic and dopaminergic systems in neuroandocrine regulation[J]. Endocrinology, 1977, 101 (2): 613-622.
  • 7Nagata M, Suzuki W, lizuka S, et al. Type 2 diabetes mellitus in obese mouse model induced by monosodium glutamate[J]. Exp Anita, 2006, 55(2): 109-115.
  • 8Scomparin DX, Grassiolli S, Gomes RM, et al. Low-Intensity swimming training after weaning improves glucose and lipid homeostasis in MSG hypothalamic obese mice[J]. Endocr Res, 2011, 36(2): 83-90.
  • 9Cohen ND, Audehm R, Pretorins E, et al. The rationale for combining GLP-1 receptor agonists with basal insulin[J]. Med J Aust, 2013, 199(4): 246-249.
  • 10林少达,陈仲贤,林楚佳,段红艳.胰高血糖素对2型糖尿病病人血浆生长激素释放肽的影响[J].中华医学杂志,2009,89(14):967-969. 被引量：4

引证文献1

二级引证文献1

中华临床医师杂志（电子版）
2010年 第7期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部