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中外4个猪种的H-FABP基因多态分析

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摘要 本研究旨在研究淳安花猪、嘉兴黑猪、金华猪和长白猪H-FABP的基因多态性。选用成年淳安花猪23头,嘉兴黑猪21头,金华猪22头,长白猪22头,取耳组织样品,用酚/氯仿法提取DNA,将PCR产物用HinfI酶切,电泳分型。结果表明,在H-FABP基因5’上游区各基因型在淳安花猪和嘉兴黑猪群体中的分布符合哈迪-温伯格平衡(P>0.05),h等位基因在淳安花猪和嘉兴黑猪中占优,而在金华猪和长白猪中是H等位基因为优势基因;第2内含子内的HinfI-RFLP位点上,嘉兴黑猪、金华猪和长白猪各基因型在群体中的分布符合哈迪-温伯格平衡(P>0.05),4个猪种中皆为B等位基因占优。结果提示淳安花猪可能有较强肌内脂肪沉积能力,为深入研究猪肌内脂肪代谢的调控机理提供基础数据。
出处 《中国畜牧兽医文摘》 2012年第11期69-70,共2页
基金 杭州市农业科学研究院青年创新基金
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