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感染禽流感病毒鸡尸体的堆肥处理与评价 被引量:5

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摘要 建造处理人工感染弱毒禽流感病毒的静态堆肥体系,考查禽流感病毒RNA的消失规律以及鸡尸的降解速率,评价利用堆肥技术处理染疫鸡尸的安全性和可行性。以木屑、鸡尸和牛粪建立静态堆肥体系,持续堆制40d。堆制期间检测温度。以H9亚型禽流感病毒攻毒的鸡,模拟禽流感爆发后病死或扑杀的染疫鸡尸,使用rRTPCR技术检测堆制过程中禽流感病毒RNA的降解情况。使用Real-timePCR方法扩增鸡源线粒体DNA,分析扩增片段的降解情况。堆肥40cm深度的温度在堆肥发酵1d后超过50℃,并保持了7d,最高温度57.5℃。禽流感病毒RNA第2d即可减少3log10,第12d的样本检测不到其存在。鸡源线粒体GH片段由8.97±0.15log10copies/g湿重降至5.35±0.30logcopies/g湿重,减少超过99%。表明所建堆肥体系能够有效灭活鸡粪中病原微生物,快速降解禽流感病毒RNA,迅速降解染疫鸡尸。
出处 《中国畜牧兽医文摘》 2012年第9期45-47,共3页
基金 自然科学基金国际重大合作项目(No.30620120430) 大连市科学技术项目(2009E11SF133)
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参考文献12

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二级参考文献2

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共引文献67

同被引文献73

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  • 10蓝卉,蔡宇杰,廖祥儒,庞建勋.一株低温脂肪酶产生菌的筛选鉴定与产酶发酵初探[J].食品与生物技术学报,2009,28(4):550-554. 被引量:6

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