丝素蛋白/羟基磷灰石复合材料修复骨缺损的微循环研究

The Study of Microcirculation on Repairing Bone Defect by Silk Fibroin/Hydroxyapatite Composite Biomaterials

了解丝素蛋白(silk fibroin,SF)表面修饰的羟基磷灰石(hydroxyapatite,HA)修复骨缺损过程中,实验动物血液流变学和骨缺损修复区血流量的变化。选择20只新西兰白兔,制作15 mm长的桡骨节段性骨缺损模型,根据植入不同移植材料分为实验组和对照组,实验组于动物左侧桡骨缺损区植入MSCs复合SF表面修饰的HA培养制备的组织工程骨,对照组植入MSCs复合HA培养制备的组织工程骨,观察各组动物术后7、14 d血液流变学和术后14 d骨缺损修复区血流量的变化。实验组与对照组比较,血液流变学指标和骨缺损修复区血流量差异显著,实验动物全身血液粘度降低,骨缺损修复区的局部血流量增加。SF表面修饰对以HA为支架材料组织工程骨的修复作用有明显优化作用。

To study the changes of hemorrheology and blood flow volume of bone defects area of experimental animals repaired by HA modified by SF.The experimental model of 15 mm radial segmental defect was produced in 20 New Zealand white rabbits which were divided into experiment and control group.Tissue engineered bone produced by MSCs cocultured with HA modified by SF was used to repair bone defect of left limbs in experiment group,while tissue engineered bone produced by MSCs cocultured with HA to repair defect in control groups.The changes of hemorrheology and blood flow volume of bone defects area were observed in different groups.The indexes of experiment group changed significantly as compared with those of control group.The blood viscosity and erythrocyte aggregation index reduced and blood flow volume of bone defect increased during the bone defects were repaired by tissue engineered bone produced by MSCs cocultured with HA modified by SF.The surface modification of SF can optimize the repairing effect of tissue engineered bone produced by HA as scaffold material.