生长季节浇肥对移栽卫矛叶片内保护酶活性的影响

The Effect of Pouring Fertilizer in Growing Season on the Protective Enzyme Activity of Leaves of Transplanted Euonymus alatus (Thunb.) Sieb.

通过对移栽卫矛苗木浇施复合肥后生长期叶片内保护酶活性变化情况的研究,判断最有利于移栽苗生长的施肥量,为实际生产提供理论支持。以3年生卫矛苗木为试材,浇施NPK比例为6:12:18的复合肥,每株施肥量为F18.33g,F228.33g,F348.33g,F468.33g,F588.33g,1个对照,3次重复。肥料平均分4个月浇入土壤,每个小区25株,随机排列。于4—8月份的每月第10天取样,测定保护酶活性。试验结果表明,SOD呈现出先升高后降低的单峰曲线变化规律,经F3、F4、F5处理后的苗木SOD比对照和F1、F2处理后的苗木SOD提前1个月达到最高值;POD活性除对照一直升高外,经施肥处理后的苗木,其POD均在6月份达到最高值后下降;对照PPO的活性在6—7月份达到最低值,而施肥后的PPO活性始终处于升高趋势,与对照变化明显不同,且7—8月的PPO活性极显著高于对照。综合比较来看,F3处理的施肥量最佳。

In order to determine the fertilizer which was most beneficial for the growth of transplanted seedlings,provide theoretical support for the practical production by studying the protective enzyme activity changes of leaves of transplanted Euonymus alatus (Thunb.) Sieb.in growing season after pouring fertilizer.This paper took 3-year-old Euonymus alatus (Thunb.)Sieb.as experiment material,poured fertilizer in which NPK ratio was 6:12:18 and the amount of fertilizer per plant for the F1 8.33 g,F2 28.33 g,F3 48.33 g,F4 68.33 g,F5 88.33 g,adopted one contrast,three repeat,poured fertilizer into the soil for 4 months on average,took 25 plants per cell and random arrangement.Then the author sampled in the 10 th day per month (April,May,June,July,August) and determine the enzyme activity.The results showed that:(a) the SOD activity changes increased and then decreased as a single-peak curve,and arrived at highest value under F3,F4,F5 treatment a month earlier than that under F1,F2 treatment;(b) the POD activity of seedlings after fertilization all arrived at the highest values in June and then decreased except the contrast;(c) the PPO activity of the contrast arrived at the lowest value in June to July,while the PPO activity after fertilization always had a increasing trend which had a significant difference with the contrast.By the comprehensive comparisons,it was found that the fertilizer under F3 treatment was the best option.