实时定量PCR分析不同栽培措施下番茄蔗糖转运蛋白(SUT1)基因表达差异

Difference of SUTI Gene Expression in Tomato under Different Cultural Measure by Quantitative RT-PCR(qRT-PCR)Reactions

通过实时荧光定量PCR进行相对定量分析,得到番茄SUT1基因在不同栽培条件下不同器官的表达差异。结果表明:叶片和果柄中SUT1基因表达量高于果实中;不同栽培措施对番茄SUT1表达量的影响各不相同,营养液的EC值较低水平时(1.8dS·m-1)SUT1基因表达量高于EC值较高水平时(3.0dS·m-1)、栽培密度较高时SUT1基因表达量高于栽培密度较低时、不摘叶时SUT1基因的表达量高于1/3摘叶及1/2摘叶,均一摘叶高于下叶摘叶。品种对SUT1基因表达量的影响比较复杂,叶片中莱邦索﹥麗容,而果柄和果实中反之,表明莱邦索比麗容更有利于蔗糖的转运和蔗糖在果实中的累积。SUT1基因在叶片中的表达量与番茄的单株产量呈正相关。

To study the differential expression of sucrose transporter(SUT1)gene under different cultural method in tomato(Lycopersicon esculentum Mill.),the experiment was performed by quantitative real time PCR(qRT-PCR)reactions.The result show that the relative expression level of SUT1 gene in leaves and stalk was higher than in fruit.The relative expression level of SUT1 gene in leaves,stalk and fruit under EC 1.8 was higher than under EC 3.0.When under bigger culture density,it was higher than under lower culture density.It was higher without clipping than with 1/3 clipping and 1/2 clipping.Influence of variety on SUT1 relative expression level was very complex.That of'Laibangsuo'in leaves was higher than'Lirong',while it was contrary to the former in stalks and fruits.This indicated that'Laibangsuo'was more favorable for sugar transportation and accumulation in fruit than'Lirong'.There was positive correlation between expression level of SUT1 gene in leaves and yield of single tomato plant.