温敏型核雄性不育系番茄T4育性的研究

Studies on Fertility of Thermo-sensitive Genic Male-sterile Tomato,Lycopersicum esculentum Mill.—T4

以温敏型核雄性不育系番茄T4及对照First为试材,通过调查花粉发芽率、自交结实率、果实种子数以及采用Tricine-SDS-PAGE电泳方法分析叶片及花药蛋白质的变化,研究T4的育性转化,并分析其在可育和不育条件下花粉发芽率、自交结实率、果实种子数、叶片及花药蛋白质的差异。结果表明:28℃/18℃(昼/夜)处理的T4花粉发芽率、自交结实率最高,果实种子数最多,与对照First差异不显著,表明此时T4恢复部分育性;28℃/24℃和28℃/12℃处理的T4花粉发芽率、自交结实率和果实种子数与对照差异均达到极显著水平,表现为不育。不同温度处理的T4花药蛋白质与叶片蛋白质差异均达到显著水平;28℃/24℃和28℃/12℃处理的T4叶片蛋白质与对照差异显著,但28℃/18℃处理的差异不明显。

Taking thermo-sensitive genic male-sterile tomato Lycopersicum esculentum Mill.T4 and the control First as experimental materials,this paper investigated pollen germination rate,self-breeding rate and fruit seed number,analyzed the protein changes in leaf and anther by Tricine-SDS-PAGE electrophoresis to comprehend fertile conversion of T4.The paper also analyzed the pollen germination rate,self-breeding rate and fruit seed number,protein differences in leaf and anther under fertility and sterility conditions.The results showed that pollen germination rate,self-breeding rate and fruit seed number of T4 were the highest with 28 ℃/18 ℃(day/night)treatment.There was no significant difference compared with the control First.It indicated that T4 had restored partial fertility.But,with 28 ℃/24 ℃ and 28 ℃/12 ℃ treatments the pollen germination rate,self-breeding rate and fruit seed number of T4 had significant differences with the control First,and T4 expressed as sterile line.There were significant differences in leaf protein and anther protein of T4 under different temperature treatments.Under 28 ℃/24 ℃ and 28 ℃/12 ℃ treatments,leaf protein of T4 was significantly different with the control First.But,there was no significant difference between T4 and the control under 28 ℃/18 ℃treatment.