补肾醒脑方对拟血管性痴呆大鼠行为学及组织内胆碱乙酰转移酶含量的影响

The Effect of Bushen Xingnao(BSXN)Prescription on Rats' Bethavior with Vascular Dementia and the Contents of Choline Acetyltransferase in Its Hippocampus Tissue

目的:观察补肾醒脑方对血管性痴呆(VD)模型大鼠海马组织胆碱乙酰转移酶(ChAT)与学习记忆功能的影响。方法:双侧颈总动脉结扎并反复缺血再灌注法制模,将大鼠分为模型组、西药组和中药组,进行药物治疗。用跳台试验检测大鼠行为学变化,用酶免法检测血清和海马组织ChAT含量。结果:补肾醒脑方组大鼠跳台检测反应期比模型组缩短(P<0.05);中药组血清和海马ChAT含量比模型组增加(P<0.05)。结论:对血管性痴呆大鼠血清和海马内ChAT含量的改善是补肾醒脑方治疗获效的机制之一。

Objective:To observe the pathology mechanism of vascular dementia(VD) rats by using Bushen Xingnao(BSXN) prescription and study the effect between hippocampal organization choline acetyltransterase(ChAT) and the function of learning and memory.Method:The VD model rats are induced by ligating two sides of the total artery in neck,repeatedly use ischemia-reperfusion method to make the models.The models are randomly divided into Chinese medicine group(CG),western medicine group(WG),model group(WG),given drug treatment.Observe VD rat models behavioral changes through using the diving platform experiment,and detect the ChAT content in serum and hippocampal organization.Results The escape latency(EL) of CG was obviously shorter than that of MG.The ChAT content of CG in serum and hippocampal organization was higher than that of MG(P<0.05).Conclusion:BSXN prescription can improve the content of ChAT in serum and hippocampal organization,which may play a key role in onset and development of VD.