生石膏对大鼠胃酸分泌及胃粘膜屏障的影响

Effects of Gypsum on Gastric Acid Secretion and Gastric Mucosal Barrier Function in Rats

目的:探讨寒性药物生石膏对大鼠胃粘膜屏障损伤的机制。方法:30只雄性SD大鼠随机分为空白对照组、生石膏灌胃组和生理盐水灌胃组。实验结束后利用激光多普勒血流仪测定胃黏膜血流量,测定动物的胃结合粘液、空腹胃液量和胃液酸度,并取各组动物的胃粘膜组织测定表皮生长因子(epi-dermal growth factor,EGF)和一氧化氮(nitric oxide,NO)含量。结果:生石膏组与对照组比较,大鼠的胃黏膜血流量及胃结合粘液量明显减少,而空腹胃液量和胃液酸度均明显增加,且胃组织内NO和EGF含量明显减少;而生理盐水组与对照组之间差异无统计学意义。结论:生石膏伤胃与其破坏胃粘膜屏障作用有关。

Objective:To investigate the mechanisms of cold-property Gypsum on gastric mucosal lesion in rats.Methods:Thirty male SD rats were randomized into three groups: control group,Gypsum gastric gavage group and NS gastric gavage group(NS).The animal's Gastric mucosal blood flow(GMBF) was measured directly by laser doppler flowmeter(LDF),the gastric wall mucus(GWM),fasting gastric juice volume and acidity were measured.The gastric mucosa levels of EGF and NO were measured.Results:Compared with the control group,the mean value of GMBF and GWM were decreased;The gastric juice volume and acidity were increased;The level of EGF and NO were great decreased in the Gypsum group.There was slightly difference between the control and the NS groups.Conclusion:The Gypsum can injure the stomach of rats,and this characteristic may be connected with its damage of gastric mucosal barrier function.