以棋盘法测定并评价汉防己甲素与酮康唑联合对白念珠菌的体外抗真菌活性

In vitro interactions between tetrandrine and ketoconazole against Candida albicans determined by chequerboard method

目的探讨汉防己甲素对酮康唑抗白念珠菌有无增效活性。方法参照美国临床和实验室标准协会(CLSI)推荐的M27-A3方案,采用棋盘式微量液基稀释法测定汉防己甲素和酮康唑联合对16株同一亲本来源的白念珠菌的体外抗真菌活性。以OD值测定法判读最低抑菌浓度,并以FICI法对联合用药结果进行评价。结果汉防己甲素与酮康唑单独作用于上述16株白念珠菌的最低抑菌浓度分别为64-128μg/mL和1-32μg/mL;两药联合时的最低抑菌浓度值分别降至2-16μg/mL和0.016-0.5μg/mL,且终点清晰,"拖尾现象"消失,FICI值为0.033-0.133,均表现为显著协同作用。结论汉防己甲素在体外对酮康唑抗白念珠菌活性有明显的增效作用。

The aim was to investigate the in vitro antifungal interactions between tetrandrine(TET) and ketoconazole(KCZ) against Candida albicans.According to the Clinical and Laboratory Standards Institute(CLSI) M27-A3 guidelines,the response was evaluated by the checkerboard microdilution method.The results of the interactions were determined by using the spectrophotometric method and the nature of the interactions was assessed by the fractional inhibitory concentration index(FICI) model.The MICs of TET and KCZ alone against 16 different C.albicans isolates were 64-128 μg/mL and 1-32 μg/mL,respectively.While the MICs of TET and KCZ in combination were 2-16 μg/mL and 0.016-0.5 μg/mL,with disappearance of the so-called 'trailing' phenomenon.The FICI of the two drugs in combination ranged from 0.033 to 0.133.Strong synergism was observed against all the 16 isolates.Our findings suggest that TET showed potent synergism when combining with KCZ.