广西空肠弯曲菌和结肠弯曲菌流行病学调查

Epidemiological investigation on Campylobacter jejuni and Campylobacter coli in Guangxi

目的了解广西空肠弯曲菌和结肠弯曲菌引起腹泻的流行病学特点以及在健康人群和动物中携带状态。方法在广西崇左市大新县和南宁市对腹泻病例、5岁以下健康人群、养猪场和屠宰厂职工以及养殖场和农贸市场的猪、鸡等进行流行病学调查并采集粪便标本,进行实验室空弯菌和结弯菌的分离培养、PCR鉴定以及脉冲场凝胶电泳分型(PFGE)分析。结果弯曲菌检出率为2.13%(21/985),空肠弯曲菌和结肠弯曲菌检出率分别为1.12%(11/985)和1.02%(10/985)。腹泻病例检出率为0.89%(6/677),以5岁以下年龄组和20～29岁年龄组检出阳性率为高,除冬季外其余季节均检出阳性;人群弯曲菌检出阳性率为8.26%(10/121),2岁以下空肠弯曲菌检出率较高,结肠弯曲菌则在每个年龄段均有检出;鸡、猪检出弯曲菌阳性率为3.23%(5/155),空肠弯曲菌和结肠弯曲菌的检出率分别为1.29%(2/155)和1.94%(3/155),两种动物的阳性率差异无统计学意义。所有生化结果阳性的菌株其PCR复核鉴定结果均为阳性。来自南宁市和大新县的两株空弯菌PFGE型别一致,提示二者可能存在相同的感染源。结论广西地区人群及部分动物中空肠弯曲菌、结肠弯曲菌的流行状况呈现多样化和复杂化态势。

The objective is to investigate the epidemiological characteristics of diarrhea caused by Campylobacter jejuni(C.jejuni)and Campylobacter coli(C.coli)and carrier rate in population in Guangxi,which will be helpful for developing control measures.Epidemiological investigation was carried out and stool samples were collected on general population younger than 5years,pig farmers,pig slaughters,as well as pigs and poultry in farms and markets.The collected specimens were cultured for C.Jejuni and C.coli.PCR and PFGE were applied to conduct identification of the Campylobacterisolats.Results showed that of the specimens tested,2.13%(21/985)were positive for Campylobacts,and 1.12%(11/985)and 1.02%(10/ 985)were positive for C.jejuni and C.coli,respectively.Among patients with diarrhea,0.89%(6/677)was positive for Campylobacter.The positive rates were higher in population under 5years and those aged 20-29years.Campylobacter was isolated in all seasons excepting for winter.Among general population,the positive rate for Campylobacter was 8.26%(10/ 121).And the highest positive rate for C.jejuni was found in population under 2years of age,while C.coli were isolated from all ages of the population.The positive rate for Campylobacterin chicken and pig was 3.23%(5/155).And the positive rates for C.jejuni and C.coli were 1.29%(2/155)and 1.94%(3/155),respectively.There was no significant difference in positive rate between these two sorts of animals.Reconfirmation with PCR was positive for all isolates which were positive for biochemical tests.It's concluded that the prevalence of C.jejuni and C.coli in population and animals was diverse and complicated.