摘要
目的建立快速准确的生物制品支原体污染检测方法。方法根据GenBank中报道的支原体基因序列,选择16SrRNA支原体种属特异性区域作为扩增区,设计合成4条引物,对样品进行检测。结果对224份样品进行PCR检测,阳性率为11.2%,培养法检出阳性13份,阳性率为5.8%。结论该方法综合PCR方法和传统培养法的优势,兼具有快速、灵敏性高、准确等优点,且能普遍应用到实践。
The aim of this study is to detect Mycoplasmaspecies in contaminated biological product rapidly.PCR primers were designed according to the published sequences of 16srRNA of Mycoplasmain GenBank.We detected 224samples by using PCR and cultivation methods,and the positive rate were 11.2%and 5.8%,respectively.In conclusion,the PCR method could identify Mycoplasmafrom biological product in a fast,sensitive and specific way,which could be widely applied in practice.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2012年第11期1152-1154,共3页
Chinese Journal of Zoonoses
关键词
支原体
生物制品
PCR
培养
污染
Mycoplasma
biological product
PCR
culture
contaminate
