丽格海棠细菌性叶斑病病原菌鉴定

Identification of Bacterial Leaf Spot Disease Caused by Xanthomonas Campestris pv. Begoniae on Rieger Begonia

2011年在云南石林丽格海棠上发现一种由细菌侵染引起的新病害,称为丽格海棠细菌性叶斑病.该病主要危害叶片,症状为叶片边缘形成倒V字型坏死和起皱.从病叶上分离到14株细菌菌株,菌株接种于不同丽格海棠品种上,发病症状与田间自然症状一致,采用柯赫氏法则重新分离得到此病的病原细菌.经培养性状、菌落形态观察、生理生化特性分析(Biolog系统)和16SrDNA序列分析以及病原菌的寄主范围测定,确定该病原菌为野油菜黄单胞菌秋海棠致病变种Xanthomonas campestris pv.begoniae.这是中国关于Xcb侵染丽格海棠的首次报道.

A new bacterial disease from Rieger begonia,which was first found in Shilin of Yunnan Province in 2011,was main harmful for Rieger begonia leaves.Initial symptoms are small circular or polygonal water-socked spot on leaf margin;later these spots expand and join together,forming bigger inverted V-shaped necrotic specks.14 strains are isolated from diseased leaves from Rieger begonia.Healthy leaves and the same strains have been re-isolated from inoculated leaves by the Koch's rule.