灵芝原生质体化学诱变育种

Research on Chemical Mutation Breeding of Protoplasts of Ganoderma lucidum

分别采用氯化锂、甲基磺酸乙酯(EMS)、亚硝酸诱变灵芝原生质体,对高产胞外多糖的菌株进行筛选。结果表明,氯化锂最佳诱变剂量为0.03%,此时诱变株L31胞外多糖产量最高为19.99 mg/mL,较原发菌株提高914.72%;EMS最佳诱变剂量为0.12%,此时诱变株E40胞外多糖产量最高为6.14 mg/mL,较原发菌株提高211.68%;亚硝酸最佳诱变时间为15 min,此时诱变株H48胞外多糖产量最高为10.13 mg/mL,较原发菌株提高414.21%;3种诱变方式下,诱变株L31的胞外多糖产量最高,且遗传性状最稳定,第三、五、七、九代胞外多糖产量较原发菌株分别提高了738.58%、671.07%、683.25%、673.10%。

Protoplasts of Ganoderma lucidum were treated by lithium chloride,EMS and nitrous acid to screen mutation strains with higher extracellular polysaccharide yield.The results showed that the optimum mutagenic dosage of lithium chloride was 0.03 % with the highest yield of extracellular polysaccharide,19.99 mg/mL,namely mutagenesis strain L31 was 914.72 % higher than original strain.The optimum mutagenic dosage of ethylmethane sulfonate was 0.12 % with the highest yield of extracellular polysaccharide,6.14 mg/mL,namely mutagenesis strain E40 was 211.68 % higher than original strain.The optimum mutagenic time of nitrous acid was 15 min with the highest yield of extracellular polysaccharide,10.13 mg/mL,namely mutagenesis strain H48 was 414.21 % higher than original strain.The mutagenesis strain L31 had the highest yield of extracellular polysaccharide and was also inherited stably.The extracellular polysaccharide yield of the third,fifth,seventh,ninth generation was 738.58 %,671.07 %,683.25 %,673.10 % higher than original strain respectively.