天冬酰胺对脂多糖刺激断奶仔猪生长性能、血细胞分类计数和血液生化指标的影响

Effects of Asparagine on Growth Performance,Blood Cell Differential Count and Blood Biochemical Indices of Weaner Pigs Challenged with Lipopolysaccharide

本试验旨在研究天冬酰胺(ASN)对脂多糖(LPS)刺激断奶仔猪生长性能、血细胞分类计数和血液生化指标的影响。选择24头"杜×长×大"断奶仔猪,随机分为4个组,每组6个重复,每个重复1头猪。4个组分别为:对照组(基础饲粮);LPS组(基础饲粮+LPS);0.5%ASN组(基础饲粮+LPS+0.5%ASN);1.0%ASN组(基础饲粮+LPS+1.0%ASN)。试验第16天,分别给LPS组、0.5%ASN组和1.0%ASN组的猪注射100μg/kg BW的LPS,对照组注射等量的生理盐水。结果表明:1)0.5%ASN组可显著缓解LPS导致的平均日增重的下降(P<0.05)。2)在4 h时,0.5%ASN组显著提高了淋巴细胞和单核细胞比例(P<0.05);1.0%ASN组显著缓解了LPS刺激导致的嗜酸性粒细胞比例的升高和血小板数量的降低(P<0.05)。在24 h时,0.5%ASN组显著缓解了LPS刺激导致的红细胞数量和红细胞压积的降低(P<0.05);1.0%ASN组显著缓解了LPS刺激导致的白细胞和嗜中性粒细胞数量的上升和淋巴细胞数量、淋巴细胞比例、血红蛋白含量及红细胞压积的下降(P<0.05)。3)在24 h时,0.5%ASN组显著缓解了LPS刺激导致的谷草转氨酶(GOT)活性的上升和葡萄糖含量的下降(P<0.05);1.0%ASN组显著缓解了LPS刺激导致的GOT活性的上升和谷丙转氨酶/谷草转氨酶值的下降(P<0.05),显著提高了总蛋白含量(P<0.05),显著降低了碱性磷酸酶活性(P<0.05)。由此可见,ASN能在一定程度上缓解LPS刺激所导致的断奶仔猪生长抑制和应激反应。

This study was conducted to investigate the effects of asparagine(ASN) on growth performance,blood cell differential count and blood biochemical indices of weaner pigs challenged with lipopolysaccharide(LPS).Twenty-four weaner piglets(Duroc×Landrace×Large) were randomly allocated into 4 groups with 6 replicates and 1 piglet per replicate.The four groups included control group(basal diet),LPS group(basal diet+LPS),0.5%ASN group(basal diet+LPS+0.5%ASN) and 1.0%ASN group(basal diet+LPS+1.0%ASN).On day 16,the piglets in the LPS group,0.5%ASN group and 1.0%ASN group were injected intraperitoneally with 100 μg/kg BW LPS,and the piglets in the control group were injected intraperitoneally with the same dose of physiological saline.The results showed as follows: 1) 0.5%ASN group significantly alleviated the decrease of average daily gain(ADG) induced by LPS(P<0.05).2) At 4 h,0.5%ASN group significantly increased the percentage of lymphocytes and monocytes(P<0.05),and 1.0%ASN group significantly alleviated the increase of eosinophilic granulocyte percentage and the decrease of platelet number induced by LPS(P<0.05).At 24 h,0.5%ASN alleviated the decrease of red blood cell number and hematokrit induced by LPS(P<0.05).1.0%ASN group significantly alleviated the increase of the number of white blood cell and neutrophili induced by LPS(P<0.05),also significantly alleviated the decrease of lymphocyte number and percentage,hemoglobin content and hematocrit induced by LPS(P<0.05).3) At 24 h,0.5%ASN group significantly alleviated the increase of glutamic oxalacetic transaminase(GOT) activity and the decrease of blood glucose content induced by LPS(P<0.05).1.0%ASN group significantly alleviated the increase of GOT and the decrease of glutamic-pyruvic transaminase(GPT)/GOT value induced by LPS(P<0.05),significantly increased the total protein content,and decreased the alkaline phosphatase activity(P<0.05).In conclusion,ASN can alleviate the growth depression and stress response induced by LPS to some degree.