盐胁迫对拟南芥种子萌发的影响

Effects of Salt Stress on Germination of Arabidopsis thaliana Seeds

为了研究盐胁迫对拟南芥种子萌发的影响,以哥伦比亚野生型拟南芥种子为试验材料,在无菌条件下将拟南芥种子分别点种在添加50、100、150、200 mmol/L的NaCl和KCl及100、200、300和400mmol/L甘露醇的MS基本培养基中进行培养;然后对拟南芥种子的萌发情况进行统计,连续统计7 d。结果表明,当NaCl、KCl和甘露醇浓度为50、50、100 mmol/L时,种子萌发率与没有盐胁迫的对照无显著差异;当NaCl、KCl和甘露醇浓度分别达到150、150和300 mmol/L时,种子萌发率均显著低于对照。随着培养基中盐浓度的提高,拟南芥种子萌发和幼苗生长所受到的抑制作用不断增强。

In order to study the influence of salt stress on seeds germination of Arabidopsis thaliana(L.) Heynh.,Columbia wild-type A.thaliana seeds were used as materials and inoculated into MS basic medium with 50,100,150,200 mmol/L NaCl,50,100,150,200 mmol/L KCl,and 100,200,300,400 mmol/L mannitol under the sterile conditions.Statistics were carried out for the seed germination rate for seven days.Results showed that when the concentration of NaCl,KCl,mannitol was 50,50,100 mmol/L,respectively,the seed germination rate was not significantly different from the control.When the concentration of NaCl,KCl and mannitol reached to 150,150 and 300 mmol/L,the seed germination rate was significantly lower than the control.With the increase of salt concentration in medium,the inhibitory effects on A.thaliana seeds germination and seedling growth were increased.