锌胁迫对铜锈环棱螺致死率、富集性、CAT和SOD活性的影响

Effect of Zinc on Lethal Rate,Accumulation,CAT and SOD Activities of Bellamya aeruginosa

以浙江省温州市瓯海茶山某河内的铜锈环棱螺Bellamya aeruginosa为受试生物,研究了不同浓度Zn2+胁迫下,铜锈环棱螺的致死效应、铜锈环棱螺对锌的富集性以及锌对铜锈环棱螺体内的过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性的影响。结果表明Zn2+对铜锈环棱螺具有较大的毒害影响。当Zn2+浓度达到3.0mg/L时,就会引起个别铜锈环棱螺死亡,死亡率为2.66%。随着Zn2+浓度的增大(0.3mg/L到2.4mg/L)和处理时间的增加(2d到8d),除个别组外,铜锈环棱螺对锌的累积量均随之上升,具有一定的时间效应和剂量效应。铜锈环棱螺在锌胁迫下,肝胰腺的CAT和SOD活性受到较大的影响。当Zn2+浓度较低时,CAT和SOD活性均有较大的升高,但当Zn2+浓度较高时,CAT和SOD活性则受到一定的抑制。

Effect of zinc on lethal rate,Zn enrichment,CAT and SOD activities of Bellamya aeruginosa under Zn stress were studied.The B.aeruginosa were collected from a river in Chashan of Ouhai,Wenzhou city,Zhejiang province.The result showed that the zinc had certain toxicity to B.aeruginosa.When the Zn2+ concentration was 3.0 mg/L,a few experimental animals died,the lethal rate was 2.66%.The Zn enrichment of B.aeruginosa were increasing when the Zn2+ concentration rises from 0.3 mg/L to 2.4 mg/L and when the treating time prolongate from 2 d to 8 d except few treating group.There are certain time-response and dose-response effects.Under the Zn stress,the CAT and SOD activities in the hepatopancreas of B.aeruginosa were affected significantly.When the Zn2+ concentrations were relatively low,there are higher CAT and SOD activities of B.aeruginosa.On the contrary,when the concentrations were relatively high,the CAT and SOD activities were restrained.