高效氯氰菊酯对草鱼肝胰脏和肾脏溶菌酶活性的影响

Effect of Beta-cypermethrin on Lysozyme Activity in Hepatopancreas and Kidney of Grass Carp

研究了暴露在不同高效氯氰菊酯浓度下的草鱼Ctenopharyngodon idella肝胰脏和肾脏溶菌酶(LSZ)的活性变化。实验中高效氯氰菊酯浓度设5组,分别为0μg/L、0.5μg/L、1.0μg/L、3.0μg/L和5.0μg/L,每组分别于1d、5d和12d取样,测定肝胰脏和肾脏溶菌酶活性。结果显示,肝胰脏LSZ活性在暴露1d、5d、12d时,各处理组均显著下降(P<0.05,P<0.01)。肾脏LSZ活性在暴露1d、5d时,0.5μg/L、1.0μg/L和3.0μg/L组显著上升(P<0.05,P<0.01),5.0μg/L组显著下降(P<0.01);暴露12d时,0.5μg/L、1.0μg/L组显著上升(P<0.05),3.0μg/L和5.0μg/L组显著下降(P<0.05,P<0.01)。表明高效氯氰菊酯对草鱼肝胰脏和肾脏具有明显的毒害作用。

The change on the activity of lysozyme in hepatopancreas and kidney of grass carp exposed to different concentration of beta-cypermethrin was researched.The concentrations of the 5 group samples of beta-cypermethrin used in the experiment were 0 μg/L,0.5 μg/L,1.0 μg/L,3.0 μg/L and 5.0 μg/L respectively.The first day,fifth day and twelfth day of each group of beta-cypermethrin were utilized to determine the activity of hepatopancreas and kidney lysozyme.Results showed that all groups of hepatopancreas LSZ activities decreased remarkably(P<0.05,P<0.01) when exposed to the first day,fifth day and twelfth day of the samples.In the case of kidney,when exposed to the first day and fifth day,the LSZ activities for the 0.5 μg/L,1.0 μg/L and 3.0 μg/L groups increased significantly(P<0.05,P<0.01).Meanwhile,the LSZ activities for the 5.0 μg/L group decreased remarkably(P<0.01).When exposed to the twelfth day,the LSZ activities for the 0.5 μg/L and 1.0 μg/L groups increased significantly(P<0.05).Accordingly,the LSZ activities for the 3.0 μg/L and 5.0 μg/L group decreased remarkably(P<0.05,P<0.01).In conclusion,beta-cypermethrin have significant toxic effect on the hepatopancreas and kidney of grass carp.