唐鱼肝脏显微和超微结构观察

Microstructure and Ultrastructure of the Liver Tissue of Adult Tanichthys albonubes

应用光镜和透射电镜对繁殖期间唐鱼Tanichthys albonubes肝脏组织的显微和超微结构进行了观察。结果显示,唐鱼肝细胞具单核,中央核仁显著;细胞质内分布着粗面内质网、线粒体、糖原颗粒和脂滴等细胞器和内含物。胆小管由2～3个相邻肝细胞质膜凹陷围成,而肝血窦则由内皮细胞的胞质、成纤维细胞等参与构成。肝细胞与周边细胞通过3种不同方式进行联系:肝细胞之间的紧密连接;与血窦的间接连接;与胆小管的邻接。这些联系方式显示了肝脏具有内分泌腺和外分泌腺功能的特点。研究还发现雌性唐鱼肝脏具有"暗"细胞和"淡"细胞两种类型。本文还讨论了唐鱼肝脏与其他硬骨鱼类肝脏一般组织结构和超微结构的异同点。

The microstructure and ultrastructure of liver of the adult Tanichthys albonubes had been investigated using light and transmission electron microscopy.The results indicated that the hepatocyte contained one nucleus.Pronounced nucleoli were observed in each nucleus.Rough endoplasmic reticulum,mitochondria,glycogen and lipid droplets were distributed in cytoplasm of hepatocytes.2~3 rosettes of hepatocytes make up the bile cannaliculi.Hepatic sinusoid was formed by endothelial cell,fibroblasts.The hepatic cells in liver have three types of conjunctions:to contact each other by close connection;to contact the sinusoid through the Disse' space;to contact the bile canaliculus with microvilli,indicating the ultrastructural characteristics as endocrine gland and exocrine gland.In this research,we provide evidence to prove that the hepatic cells of the adult T.albonubes can be divided into two types:light cell and dark cell.Difference of the histological structure and ultrastructure of the liver between adult T.albonubes and other teleosts were also been discussed in this paper.