柱型苹果GRAS基因家族MdSCR的克隆与生物信息学分析

Cloning and Bioinformatic Analysis of MdSCR Gene of GRAS Gene Family in Columnar Apple

以"富士×特拉蒙"杂交后代柱型株系新梢为试材,用同源克隆方法,得到了柱型苹果GRAS基因家族的MdSCR基因,开放阅读框(ORF)长度为1458bp,编码486个氨基酸,推测其蛋白质分子质量为54.894kD,等电点(pI)为5.96,具有GRAS基因家族典型结构域。MdSCR基因与毛果杨(Populus trichocarpa)GRAS转录因子的氨基酸序列具有67.8%的相似性,与江南卷柏(Selaginella moellendorffii)、葡萄(Vitis vinifera)、大豆(Glycine max)的SCARECROW-like氨基酸序列均有58.5%以上的相似性。氨基酸聚类分析表明,苹果和毛果杨、江南卷柏聚类关系最近,其次是葡萄,关系最远的是欧洲赤松;生物信息学分析表明,MdSCR的蛋白质二级结构有208个α-螺旋,54个延伸链和224个无规则折叠区。

The shoot tips of columnar apple progeny of 'Fuji×Telamon' were used as experimental materials for cloning MdSCR gene,which belonged to GRAS transcription factors gene family.The open reading flame(ORF) of MdSCR was 1464bp,encoding 486 amino acids,and the estimated molecular weight and isoelectric point(pI) of the putative protein were 54.894 kD and 5.96.The results indicated that MdSCR had highly conserved GRAS domain.MdSCR has 67.8% amino acid sequence similarity with GRAS transcription factors of Populus trichocarpa,and has more than 58.85% amino acid sequence similarity with GRAS transcription factors of Selaginella moellendorffii,grape and soybean.Amino acids cluster analysis showed that apple and Populus trichocarpa,Selaginella moellendorffii had the closest clustering relations,and followed by grape,the furthest was Pinus sylvestris.Bioinformatics analysis showed that the protein secondary structure of MdSCR has 208 alpha helix,54 extended strand,and 224 random coils.