多世代高油酸花生示踪研究

Study on High Oleic Acid Peanut by Detecting Oleic Acid Content of Multiple Generations

本试验以2个普通油酸含量和2个高油酸含量的花生品种为亲本杂交获得2个衍生群体R2001(花育22号×06B16)和R8001(花育28号×P76),采用近红外无损伤测定方法测定R2001(F2、F3、F5、F6)和R8001(F2、F4、F5、F6、F7)各世代对应(单株)株系的油酸含量,分析不同世代间的油酸变化情况及关联情况。结果表明:群体R2001和R8001遗传上有差异,R2001 F6代高油酸与低油酸个体比例符合11,R8001 F7代高油酸与低油酸个体比例符合13;主要分离世代为F2和F3代,从F4代开始各株系的油酸含量基本稳定;R2001群体低世代油酸含量介于55%～70%的个体和R8001群体低世代油酸含量介于65%～75%的个体处于疯狂分离状态。

In the present study,two populations R2001 derived from a cross of Huayu22×06B16 and R8001 derived from a cross of Huayu28×P76 were selected as materials.In orde to analyse oleic variation and relation among generations,we detected oleic acid content of all materials including parents,R2001(F2、F3、F5、F6) and R8001(F2、F4、F5、F6、F7)by near infrared spectroscopy(NIRS).The results showed that the No.of individuals with high oleic acid and low oleic acid for R2001 population was segregated at ratio of 11 and at ratio of 13 for R8001 population.Oleate trait were mainly segregated in F2 and F3 generations.Since F4 generation,the oleic acid content of high generations was relatively stable.These individuals with oleic acid content from 55% to 70% in R2001 low generation and oleic acid content from 65% to 75% in R8001 low generation were segregated severely.