氧致视网膜病变鼠血清及视网膜PEDF和VEGF不同时程表达的研究

Different expression of VEGF and PEDF in serum and retina of oxygen-induced retinopathy rat during the making modes

目的探索血清及局部视网膜组织中PEDF、VEGF的变化在氧致视网膜病变大鼠中的作用。方法将48只7D龄SD大鼠新生乳鼠分为实验组与对照组。实验组置于浓度为75%的高氧环境中生活5d后,返回正常环境饲养,对照组于正常环境中饲养。17d及22d龄时分别行视网膜造影铺片,苏木精-伊红染色计数突破内界膜内皮细胞数,免疫组化检测视网膜PEDF、VEGF的表达,血清酶联免疫吸附试验(ELASA)检测两者血清的表达。结果实验组分别与相应对照组相比,视网膜呈缺血缺氧性增殖性表现,突破视网膜内界膜的内皮细胞数明显增加,差异有统计学意义(P<0.01);17d龄模型组视网膜VEGF染色明显增强,阳性率增加(P<0.01);22d龄模型组PEDF染色较17d龄减弱,阳性率减少(P<0.01);各组血清VEGF浓度差异无统计学意义(P>0.05);17d龄模型组血清PEDF浓度较17d正常组明显减少(P<0.01),22d龄模型组较17d龄血清PEDF增加(P<0.01)。结论血清内PEDF浓度的明显降低,视网膜组织局部VEGF的异常高表达,使血清及局部组织内PEDF/VEGF平衡的破坏可能是视网膜血管增殖性变化的重要机制。

Objective To study the effection of the variation in the balance of VEGF and PEDF in serum and retina of oxygen-induced retinapathy rat.Methods A total of 48 postnatal day 7 SD rats were randomly assigned to either experimental group(10 cases) or control group(10 cases).The experimental group was exposed to 75% oxygen for 5 days,and then in room air for ####