摘要
目的观察人乳腺癌及癌旁组织中SIAH(seven in absentia homologues)2的表达情况,探讨SIAH2在乳腺癌及癌旁组织差异表达的临床意义。方法应用免疫组织化学检测110例乳腺癌及癌旁石蜡包埋组织中SIAH2,Western blotting和Realtime PCR检测35例乳腺癌及癌旁组织中SIAH2的蛋白表达情况。结果乳腺癌组织中SIAH2蛋白表达量明显高于癌旁乳腺组织中SIAH2蛋白表达量(P<0.05);乳腺癌组织中SIAH2mRNA表达量明显高于癌旁乳腺组织中SIAH2mRNA蛋白表达量(P<0.05)。结论 SIAH2过表达与乳腺癌密切关系。SIAH2可能成为防控乳腺癌的靶点。
Objective To explore the clinical significance of SIAH2(seven in absentia homologues) in breast carcinoma and adjacent tissues.Methods The SIAH2 expressions of all the 110 cases were detected by immunohistochemistry in whom 35 cases were randomly selected to evaluate the expression of SIAH2 by RT-PCR and Western blot methods.Results The expression of SIAH2 protein and mRNA in breast carcinoma was significantly higher than that of adjacent breast tissue(P<0.05).Conclusion There was a close relationship between higher SIAH2 expression and breast cancer.The SIAH2 may be a new target for breast carcinoma therapy.
出处
《岭南现代临床外科》
2012年第6期403-406,共4页
Lingnan Modern Clinics in Surgery
