摘要
建立了帕那珠利原料药含量测定的HPLC法。以0.2%乙酸水溶液-乙腈(43∶57)为流动相,采用Diamonsil C18反相色谱柱(250 mm×4.6 mm,5μm),柱温35℃,流速1 mL/min,紫外检测波长255 nm。在此实验条件下,帕那珠利在10~100μg/mL浓度范围内与峰面积线性关系良好(r=0.9999,n=5);精密度良好,RSD为0.25%(n=5);平均回收率为98.6%,RSD为1.2%(n=9);重现性良好,RSD为0.69%(n=5);样品在24 h内稳定,RSD为0.74%。本方法准确、简便、快速,可以用于帕那珠利的含量测定。
The paper established an HPLC method for determination of ponazuril.The diamonsil C18 column was used;the mobile phase consisted of water(0.2% acetic acid)-acetonitrile(43∶ 57);the flow rate was 1 mL/min;the column temperature had been remained at 35 ℃;the detection wavelength was 255 nm.The linear range of ponazuril was 10~100 μg/mL(r=0.9999).The mean recovery was 98.6%(n=9).The precision was excellent(RSD=0.25%,n=5) as well as the reproducibility(RSD=0.69%,n=5).The sample was stable enough to be detected in 24 hours(RSD=0.74%).This method is accurate,easy and fast.It can be used to detect and quantify ponazuril.
出处
《中国兽药杂志》
2012年第8期35-38,共4页
Chinese Journal of Veterinary Drug