HPLC法测定大鼠血浆中甲硝唑的浓度

Determination of Metronidazole in Rat Plasma by High-performance Liquid Chromatography

目的建立大鼠血浆中甲硝唑浓度测定的HPLC法.方法选用Agilent Zorbax Eclipse XDB-C18色谱柱,流动相为甲醇-乙腈-水-冰乙酸(10:10:80:0.1),流速为1.0 mL/min,紫外检测波长为317 nm,柱温25℃,以替硝唑作内标物.结果甲硝唑的线性范围为1～1 000 mg/L,呈良好的线性关系(r=0.996 9),在低、中、高3个质量浓度的准确度分别为99.69%、96.35%、101.2%.RSD分别为0.3%、6.92%、8.66%,其日内、日间精密度≤15%.结论可用于甲硝唑在大鼠体内的药物动力学研究.

Objective To develop a method for determination of metronidazole and tinidazole in rat plasma.Methods A Agilent Zorbax Eclipse XDB-C18 column was used at column temperature of 25 ℃,and the mobile phase contained methanol-acetonitrile-water-acetic-acid(10:10:80:0.1,V:V),with tinidazole as the internal standard.The analyte was determined by UV at 317 nm.Results Metronidazole show good relation at range of 1～1 000 mg/L(r =0.9969),the accuracy of metronidazole were 99.69%,96.35%,101.2%.RSD were 0.3%,6.92%,8.66%.The precision RSD was less than 15 %.Conlcusions The method has been proved to be a simple and sensitive method with good precision and accuracy.It is suitable for studying pharmacokinetics of metronidazole in rats