低氧诱导因子-1α和环氧合酶-2在豚鼠银屑病样模型皮损中的表达及意义

Expression of hypoxia-inducible factor-1α and cyclooxygenase-2 in epidermis of guinea pig psoriasis-like animal model

目的研究低氧诱导因子-1α(HIF-1α)和环氧合酶-2(COX-2)在豚鼠银屑病样动物模型皮损表皮中的表达,探讨其在银屑病发病机制的作用。方法用5%普萘洛尔乳剂外涂豚鼠耳背部皮肤诱导银屑病样动物模型,应用免疫组化法检测正常豚鼠表皮和豚鼠银屑病样动物模型皮损表皮中HIF-1α和COX-2表达。结果 5%普萘洛尔外涂所致豚鼠银屑病样动物模型皮损中HIF-1α和COX-2表达水平较正常对照组明显增高,有显著性差异(P均<0.05)。结论 HIF-1α和COX-2在豚鼠银屑病样动物模型皮损的表皮中表达明显增高,不仅在组织病理学而且在分子水平上与人类银屑病皮损基本一致。

Objective To study hypoxia-inducible factor-1α(HIF-1)and cyclooxygenase-2(Cox-2)expression in epidermis of guinea pig psoriasis-like animal model and their roles in the pathogenesis of psoriasis.Methods Psoriasis-like animal model was established by topical treatment with 5% propranolol emulsion on ear′s back of guinea pig.We detected HIF-1α and Cox-2 in normal epidermal tissues from 20 normal guinea pig and the lesions of ear′s back from guinea pig psoriasis-like animal model by in immunohistochemistry.Results Expression of HIF-1α and Cox-2 in lesions of treated group were significantly increased,while in normal epidermis there were no positive staining expression.Compared with the normal epidermis,the positive rate and the mean expression level of HIF-1α and Cox-2 in epidermis of guinea pig psoriasis-like animal model were significantly higher than those in the normal epidermal(P<0.05).Conclusions The expression of HIF-1α and Cox-2 in the epidermis of guinea pig psoriasis-like animal model were higher than normal epidermal.It has high concordance with human psoriatic lesions not only in histopathology,but also at molecular level.