异丙酚预处理诱导PC12细胞缺氧耐受作用研究

The protective effect of propofol preconditioning induced tolerance against hypoxic injury in PC12 cells

目的观察异丙酚预处理对PC12细胞缺氧损伤的影响。方法对数生长期的PC12细胞分为四组:对照组(C组),普通培养箱内常规培养;脂肪乳剂组(F组),在细胞培养基中加入10%脂肪乳剂,置普通培养箱内常规培养;缺氧组(H组),细胞置三气培养箱内培养,设置培养条件为2%O2、5%CO2;异丙酚+缺氧组(PH组),在细胞培养基中加入终浓度为2mmol/L的异丙酚,再置入三气培养箱培养,条件设置同H组。四组细胞均在培养4h后行细胞活力和细胞凋亡检测。结果 C组与H组之间细胞活力和细胞凋亡率的差异有统计学意义(P<0.05);与H组相比,PH组细胞活力明显增高(P<0.05),细胞凋亡率明显降低(P<0.05)。结论异丙酚预处理改善PC12细胞活力,通过抑制细胞凋亡诱导PC12细胞缺氧耐受。

Objective To investigate the effect of propofol preconditioning on hypoxic injury in PC12 cells.Methods PC12 cells in logarithmic phase were randomly assigned to 4 groups:control group(C group),general cultured in common incubator;fat emulsion group(F group),10% fat emulsion was added in culture medium and general cultured in common incubator;hypoxic group(H group),PC12 cells were cultured in three gas incubator,set the culture condition as 2% O2,5% CO2;propofol+hypoxic group(PH group),2 mmol/L propofol was added in culture medium,then cultured in three gas incubator,the culture condition was same as H group.Assay of MTT and apoptosis were performed after 4 h in culture.Results The difference in cytoactive and ratio of apoptotic cells between C group and H group had statistical significance(P<0.05);compared with H group,the cytoactive of PH group increased obviously(P<0.05),and the ratio of apoptotic cells decreased obviously(P<0.05).Conclusions Propofol preconditioning could improve the cytoactive of PC12 cells,and induce tolerance against hypoxic injury in PC12 cells by inhibiting apoptosis.