CD133在未分化甲状腺癌肿瘤干细胞中的作用研究

The study on the relationship between CD133 and the cancer stem cells of anaplastic thyroid carcinoma

目的探讨CD133与未分化甲状腺癌肿瘤干细胞的联系,为新的治疗方向提供研究基础。方法利用流式细胞技术分析未分化甲状腺癌ARO细胞株中的CD133阳性百分比,采用磁珠分离技术分离CD133阳性及CD133阴性亚群细胞,分别用RT-PCR技术检测OCT-4基因表达,并观察其肿瘤细胞球的形成。结果 CD133阳性细胞在未分化甲状腺癌ARO细胞株中占8.9%,CD133阳性细胞的OCT-4蛋白表达明显高于CD133阴性细胞(P<0.05),且更容易形成肿瘤细胞球,提示CD133阳性细胞的体外增殖能力比CD133阴性细胞强,具有肿瘤干细胞的特性。结论 CD133阳性可能是未分化甲状腺癌的肿瘤干细胞标记物之一。

Objective To explore the relationship between CD133 and the cancer stem cells of anaplastic thyroid carcinoma.Methods The ARO cells of anaplastic thyroid carcinoma were determined by flow cytometric analysis,isolated into CD133 positive and CD133 negative subpopulation by magnetic beads,which were detected the RNA expression of OCT-4 by RT-PCR and observed the sphere formation individually.Results CD133 positive cells accounted for 8.9% in ARO cells,the expression of OCT-4 was higher than that in CD133 negative cells(P<0.05),and seemed to form spheres.It is indicated that the proliferiation ability of CD133 positive cells was higher than that of CD133 negative cells in ARO cells in vitro,which was one of the properties of cancer stem cells.Conclusions It was suggested that CD133 positive might be one of the surface markers of cancer stem cells in anaplastic thyroid carcinoma.