顶空毛细管气相色谱法测定交联透明质酸钠凝胶中的残留溶剂

Determination of Residual Solvents in Cross-linked Sodium Hyaluronate Gel by CGC

采用顶空毛细管气相色谱法测定交联透明质酸钠凝胶中的残留溶剂。采用SE-30毛细管柱(50 m×0.53 mm×3.0μm),FID检测器,检测器温度为250℃,进样口温度为180℃;柱温为50℃,保持2 min,以10℃/min的升温速率升到160℃,保持5 min;顶空进样,平衡温度为80℃,平衡时间为30 min,分流比为1∶1;溶剂为水。被测物均能得到很好的分离,峰面积与质量浓度线性关系良好,精密度和回收率良好。该方法可用于交联透明质酸钠凝胶中残留溶剂的测定。

To establish a method for the determination of residual solvents in cross-linked sodium hyaluronate gel with head-space capillary gas chromatography(CGC),a SE-30 capillary column(50 m×0.53 mm×3.0 μm) and hydrogen flame ionization detector(FID) were applied.The detector temperature was 250℃ and the injector temperature was 180℃.The column temperature was maintained at 50℃ for 2 min,then raised at a rate of 10℃ / min to 160℃ and maintained for 5 min.The sample was dissolved in water.The containers of head-space injector were in equilibrium at 80℃ for 30 min,and the splitting-ratio was 1∶1.The measured solvents were separated completely.The calibration curve of each solvent showed a good linear relationship with good precision and accuracy.