基于神经血管单元和网络药理学理论的抗脑卒中神经病变靶点分析

Study about target-network of anti-cerebral infarction neuropathy based on theory of neurovascular unit and network pharmacology

目的:总结抗脑卒中神经病变的潜在药物靶点,为开发治疗脑卒中神经病变的创新组分中药提供资料。方法:根据国内外文献总结了脑缺血再灌注损伤导致神经血管单元的3种细胞即神经元、神经胶质细胞和脑微血管内皮细胞死亡和凋亡的信号机制。结果:重要通路有5个:炎性因子-MMPs通路-Caspases,Ca2+-线粒体通路-Caspases,Ca2+-磷脂酶-PI-3K/AK通路,Ca2+-自由基-MAPK通路,Ca2+-NO-蛋白酶通路。其中重要节点是Caspases,Ca2+和NO。结论:针对脑卒中神经病变信号网络的关键环节,发挥中药多层次多机制的特色,在神经血管单元和网络药理学理论指导下的创新组分中药开发极具价值。

Objective:Potention drug-targets on anti-neuropathy of stroke were summarized,and it will provide materials for developing innovation components traditional Chinese medicine on anti-cerebral infarction neuropathy.Method:This article had done a series of researching work about neurovascular unit which includes three kinds of cells:neuron,gliacyte,brain microvascular endothelial cell,then signal mechanism of cell death or apoptosis of each section of stroke neuropathy was analysised by the historical documents.Result:There are five important pathways:inflammatory factor-MMPs pathway-Caspases,Ca2+-mitochondrial pathway-Caspases,Ca2+-Phospholipase-PI-3K/AK pathway,Ca2+-radical-MAPK pathway,Ca2+-NO-protease pathway,among all the nodes,Caspases,Ca2+,No were the most important ones.Conclusion:Developing the multi-mechanism and multilevel of traditional chinese medicine under the guidance of the theories of network pharmacology and neurovascular unit will play an important role in studying the key links of signal-network of stroke neuropathy.