摘要
目的:观察血红素加氧酶(HO)-1在大承气汤(DD)改善脂多糖(LPS)所致小鼠急性肺损伤(ALI)中的作用。方法:将75只雄性昆明种小鼠随机分为对照组、LPS组(经气管内滴注LPS复制ALI模型)、DD+LPS组、DD+LPS+ZnPP(锌原卟啉,HO-1特异性阻断剂)组和DD组。各组小鼠鼠于给药后6 h处死。测定肺系数;光镜观察肺组织形态学改变;检测支气管肺泡灌洗液(BALF)的中性粒细胞(PMN)数目和蛋白含量的变化;采用RT-PCR和Westernblot方法检测肺组织中HO-1 mRNA和蛋白表达的变化。结果:气管内滴注LPS可引起小鼠肺组织明显的形态学改变;BALF中PMN数目和蛋白含量均增加。肺组织中HO-1 mRNA和蛋白表达均增加。预先给予DD再气管内滴注LPS,肺组织损伤减轻,BALF中PMN数目和蛋白含量均减少,而肺组织中HO-1 mRNA和蛋白表达与LPS组相比均增加。HO-1抑制剂ZnPP可抑制DD改善肺损伤的作用。结论:DD改善LPS所致小鼠ALI的作用与其可上调HO-1 mRNA和蛋白表达有关。
To explore the role of heme oxygenase(HO)-1 experimental system in dachengqitang(DD) ameliorating ALI induced by lipopolysaccharide(LPS) in mice.Seventy-five male Kunming mice were randomly divided into control group(normal saline was instilled intratracheally(50 μL/per mouse),LPS group(LPS was instilled intratracheally to replicate ALI model),DD+LPS group,DD+ LPS+ZnPP(ZnPP,HO-1 specific inhibitor) group and the DD group.Mice were killed at 6 h after administration.Lung indexes were tested;lung histomorphological changes were observed under microscope,and neutrophils(PMN) number and protein content of bronchoalveolar lavage fluid(BALF) were measured;HO-1 mRNA and protein expression in lung tissue were detected by RT-PCR and Western blot.The results showed that intratracheal instillation of LPS in mice can cause significant morphological changes in lung tissue.Both PMN numbers and protein content in BALF were increased.meanwhile the expressions of HO-1mRNA and protein in Lung tissue were increased.Pretreated with DD and then intratracheally instillated LPS coulde ameliorat lung tissue injury,reduced PMN BALF number and protein content,but increase HO-1mRNA and protein expression in the lung tissue when compared with LPS.HO-1 inhibitor ZnPP coulde inhibite the ameliorative effect of DD.The results suggest that the ameliorative effect of DD on ALI induced by LPS in mice were related with upregulation HO-1 mRNA and protein.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第2期250-254,共5页
China Journal of Chinese Materia Medica
基金
河北省科学技术研究与发展计划基金项目(06276102D-41)
