摘要
目的:建立苦荞麦总黄酮含量测定方法。方法:应用L9(34)正交试验设计优选苦荞麦总黄酮的最佳提取工艺条件。采用紫外-可见分光光度法测定总黄酮含量。结果:优选所得的苦荞麦总黄酮最佳提取工艺条件为:加100倍量甲醇,加热回流2次,每次提取2 h。苦荞麦总黄酮平均含量为4.03%,RSD为0.98%(n=5);平均回收率为100.98%,RSD为2.15%(n=5)。结论:优选所得提取工艺条件稳定可行。用紫外-可见分光光度法测定苦荞总黄酮含量,稳定性好、合理、可行。
Objective: To investigate the determination method of the total flavonoids content in Fagopyrum tartaricum (L.) Gaertn. Method: The optimum extraction condition for Fagopyrum tartaricum(L.)Gaertn was investigated by the L 9 (3 4 ) orthogonal design. The content of the total flavonoids was determined by UV-VIS. Result: The optimal conditions of the total flavonoids content for Fagopyrum tartaricum(L.)Gaertn were as follows: the raw materials were extracted 2 times with 100 times methanol for 2h. The average total flavonoids content was 4.03%, and the RSD was 0.98% (n=5). And the average recovery rate was 100.98%, and the RSD was 2.15% (n=5). Conclusion: The optimized condition of extraction process is stable and feasible. The determination method with UV-VIS is reliable, reasonable and feasible.
出处
《中药与临床》
2012年第6期23-25,共3页
Pharmacy and Clinics of Chinese Materia Medica
