摘要
以显脉金花茶幼嫩带芽茎段作为外植体,开展外植体灭菌、启动培养及增殖培育试验,结果表明:3月采集的外植体最易灭菌,其最佳灭菌方案为先用75%酒精浸泡20 s后用0.1%升汞浸泡9 min;最佳启动培养基配方为WPM+6-BA 7 mg.L-1+NAA 0.25 mg.L-1;最佳增殖培养基配方为:改良WPM(NO3-∶NH4+=2∶1)+6-BA 5 mg.L-1+NAA 0.05 mg.L-1。
Taking Camellia euphlebia young and tend stems with buds as explants,the explant sterilization was developed,together with the primary culture and multiplication culture experiment,the results showed that: explants collected on March were easy to sterilize with program of soaking with 75% of alcohol for 20S and later soaking with 0.1% of mercuric chloride for 9min;the culture medium formula for the best initiation was WPM + 6-BA 7 mg.L-1 + NAA 0.25 mg.L-1;the best formula for the proliferation medium was modified WPM(NO3-: NH4+ = 2∶ 1) + 6-BA 5 mg.L-1 + NAA 0.05 mg.L-1.
出处
《福建林业科技》
北大核心
2013年第2期73-77,共5页
Journal of Fujian Forestry Science and Technology
关键词
显脉金花茶
外植体灭菌
启动培养
增殖培养
Camellia euphlebia
explant sterilization
primary culture
multiplication culture
