脂蛋白磷脂酶A2基因V279F和A379V多态性与老年冠心病的关系

Association of V279F, A379V polymorphism in lipoprotein-associated phospholipase A2 gene with elderly coronary heart disease

目的探讨脂蛋白磷脂酶A2基因V279F和A379V多态性位点与老年冠心病(CHD)患者遗传易感性的关系。方法纳入227例CHD患者和101例正常对照者,应用DNA测序仪测脂蛋白磷脂酶A2基因V279F和A379V的基因多态性。结果 (1)CHD组V279F和A379V的基因型和等位基因频率与对照组无明显差异;(2)心肌梗死组V279F和A379V的基因型等位基因频率与非心肌梗死组亦无明显差异。(3)logistic回归分析未能发现V279F和A379V变异是CHD的危险因素。结论脂蛋白磷脂酶A2基因V279F和A379V多态性与中国汉族老年人口CHD无明显相关性。

Objective To investigate the association of V279F and A379V point mutation of lipoprotein-associated phospholipase A2 (Lp-PLA2) with coronary heart disease (CHD) in the elderly patients. Methods 227 patients with CHD and 101 controls were genotyped by DNA sequencing instrument. Results There were no significant differences between CHD group and control group in the frequencies of V279F and A379V genotypes and allele, but the frequency of the 379V allele was significantly higher in the patients with myocardial infarction(MI) than that in the patients without MI (P<0.05). Binary logistic regression analysis demonstrated that polymorphism of V279F and A379V was not an independent risk factor for CHD. Conclusions The V279F and A379V polymorphism of Lp-PLA2 gene has no significant correlation with CHD in the elderly patients, while 379V allele may increase the risk of MI in the Chinese Han elderly population.