高效石油降解菌的筛选、鉴定及其配比优化的研究

Study on the Isolation and Identification of Petroleum-Degrading Strains and Its Ratio Optimization

从克拉玛依地区石油污染土壤中分离筛选出4株高效石油降解菌S1、S2、S5和S8,经形态观察、生理生化反应和分子鉴定,确定4株菌分别为蜡样芽孢杆菌(Bacillus cereus)、恶臭假单胞菌(Pseudomonas putida)、枯草芽孢杆菌(Bacillus subtilis)和地衣芽孢杆菌(Bacillus licheniformis)。为了提高对石油的降解效率,对4株菌的添加比例进行了响应面的优化。结果表明,当石油含量为1.5 g时,菌种S1、S2、S5和S8接种量分别为0.21 g、0.22 g、0.41 g和0.22 g时的石油降解率达到最大值。在该条件下石油降解率预测值为60.17%,验证值为60.10%。

Four strains(S1,S2,S5,S8),with good ability of degrading oil,are isolated from oil-contamination soil of Karamay areas.Based on morphologic observation,physiological and biochemical characteristics,molecular identification,they are identified as Bacillus cereus,Bacillus licheniformis,Bacillus subtilis and Pseudomonas putida respectively.In order to improve the efficiency of the degradation,the ratio of four strains is optimized by using response surface methodology.The result shows that the optimum conditions are as follows:when the oil content is 1.5 g and the inoculation quantity of the four strains(S1,S2,S5,S8)is 0.21 g,0.22 g,0.41 g,0.22 g,respectively,the rate of oil degradation can reach the maximum.Under these conditions,the predictive value of oil degradation is 60.17%,meanwhile the verification value is 60.10%.