摘要
北京棒杆菌CICC21721经亚硝基胍(NTG)诱变,涂布在含有不同浓度链霉素的完全培养基上,对生长出来的菌落进行摇瓶发酵并测定赖氨酸、乳酸、残糖含量。赖氨酸产酸最高的一株突变株产量为40.2g/L,较出发菌株提高了12.2%,糖酸转化率达到58.7%。
L-lysine over producing mutants were derived from corgnedacterian pekinense CICC21721 by means of mutations with NTG.The mutants were selected directionally from the agar plates with different concentration of streptomycin and detect the L-lysine, lactic acid yield and reduced glucose in shake flasks. The best mutant strain could accumulate L-lysine up to 40.2 g/L, which is 12.2% higher than the original strain, and with a conversion of 58.7%.
出处
《发酵科技通讯》
CAS
2013年第2期9-12,共4页
Bulletin of Fermentation Science and Technology
关键词
北京棒杆菌
链霉素抗性
诱变
发酵
赖氨酸
corgnedacterian pekinense
streptomycin resistance
mutagenesis
fermentation
L-lysine
