一株新的高效解无机磷菌LNUT07的分离鉴定及其生物学特性的初步研究

Solation and Identification of a New Efficient Inorganic Phosphorus-solubilizing Bacteria LUNT07 and Its Preliminary Research of Biological Characteristics

对采自辽宁省沈阳市郊区的棚室土壤样品,开展解无机磷细菌分离纯化和筛选的研究.根据解磷圈直径和菌落直径比值大小,通过平板法初步筛选到了7株解无机磷细菌,进一步根据钼锑抗比色原理通过液体摇瓶培养法复筛出1株解无机磷能力最强的菌株LNUT07,测定其解磷能力,高达796.487mg/L,同时对该菌株进行了形态特征观察、生理生化特性、BIOLOGGN测定、16SrDNA序列分析等一系列试验,初步鉴定其为鲍氏不动杆菌(Acinetobacter baumannii).

We got the soil samples from the greenhouses in the suburbs of Shenyang,Liaoning Province to do some researchs about separation and purification of inorganic phosphorus- solubilizing bacteria. According to the solubilizing circle diameter and the colony diameter ratio, w e initially got 7 inorganic phosphorus-solubilizing bacterium strains through the plate method. And then in the shake culture rescreening experiment. by Mo-Sb colorimetric principle,one is the best( LUNT07) ,w hich show s the highest inorganic phosphorus-solubilizing ability,up to 796. 487 mg / L. It is identified as Acinetobacter baumannii by a series of experiments such as morphological characteristics,physiological and biochemical characteristics identification,the analysis of BIOLOG GN determination and the 16 S rDNA sequences tests.