乳腺癌细胞中脱氧核糖核酸含量与ER及PR表达之间的关系

Breast cancer cell DNA content and ER and PR expression

目的探讨乳腺癌细胞中脱氧核糖核酸(DNA)含量与雌激素受体(ER)、孕激素受体(PR)之间的相互关系及意义。方法从104例乳腺癌中筛选出符合DNA检测和免疫标记标准的病例35例,并对该组进行乳腺癌细胞中DNA含量、ER、PR表达的研究。结果该组乳腺癌病例发病年龄29～84岁,平均56.5岁,占104例乳腺癌3.36%。形态上,ER及PR免疫组化染色阳性结果主要为肿瘤细胞核着黄褐色,但也有部分胞浆着色,在35例肿瘤组织中,19例ER阳性,阳性率54.28%。14例PR阳性,阳性率40.0%。其中各型乳腺癌的阳性及DNA含量测定可根据本文表1～2明示。结论本研究结果发现,乳腺癌ER和PR的阳性率与肿瘤组织的分化程度,组织学分级和临床分期之间无明显差异,说明ER和PR阳性表达与肿瘤的形态学结构无明显相关,只是表明该肿瘤具有激素依赖性特征。同时研究结果表明检测DNA含量是预知肿瘤预后的一种独立的有效的方法。

Objective To explore the relation of breast cancer cells DNA content and estrogen receptor(ER) and progesterone receptor(PR) expression.Methods In 35 patients,the breast cells DNA and ER and PR expression were determined.Methods Using immunohistochemical staining,19 cases of ER were positive(positive rate 54.28%),14 cases of PR were positive(positive rate 40.0%).Conclusion It is found that the breast cancer ER and PR positive rate and the differentiation degree,histological grade and clinical stage have no obvious relation.At the same time,the results of the study show that detection of DNA content can predict the prognosis of tumor.