摘要
以细辛的根状茎为外植体进行组织培养及继代培养、适合愈伤组织诱导并增殖的培养基为MS+6-BA3.0 mg·L^(-1)+NAA mg·L^(-1);适合诱导再生植株并快速增殖的培养基为MS+6-BA0.8mg·L^(-1)+NAA0.1mg·L^(-1);生根培养基为MS+NAAO.2 mg·L^(-1)+0.5％活性炭。经生根壮苗培养30d左右,移栽后成活率可达98％以上。
The phizome of Asarum Slboldii Mig was used as tissue culture explant. The optimal media
were: (1) MS+6-BA3mg. L^(-1)+NAA0. 1mg. L^(-1) for callus induction and subculture; (2) MS+6-
BA0. 8mg. L^(_1)+NAA0. 1mg. L^(-1) for adventitious bud induction and rapid propagation; (3) MS+
NAA2mg. L^(-1) and 0. 5% active charcoal for shoot rooting. The shoot was planted to the seedbed after
rooting culture for 30 days. The survival rate of transplanting was about 98%.
出处
《佛山科学技术学院学报（自然科学版）》
CAS
2004年第2期67-68,共2页
Journal of Foshan University(Natural Science Edition)
基金
佛山科技发展专项基金(N0.0102009A)
关键词
细辛
组织培养
快速繁殖
Asarum siboldii Miq.
tissue culture
rapid propagation
