丙型肝炎病毒MHC-Ⅱ类抗原表位预测

Prediction of MHC-Ⅱ epitopes of hepatitis C virus

目的预测各型丙型肝炎病毒(HCV)的MHC-Ⅱ类抗原表位,为HCV的疫苗研发提供理论依据。方法从NCBI数据库中检索出10条包含6种基因型的HCV完整氨基酸序列,使用在线MHC-Ⅱ类抗原表位分析工具NetMHCⅡpan-2.1 Server,从MHC-Ⅱ类抗原表位在6种HCV型别之间的保守性及与MHC-Ⅱ类分子亲和力两方面评价HCV携带的潜在MHC-Ⅱ类抗原表位。结果位于NS3和NS5B蛋白的3段表位核心序列具有较好的保守性,其中尤以NS3蛋白携带的表位核心序列保守性最好;高亲和力的MHC-Ⅱ类抗原表位主要位于E2、NS5A、NS4A和NS5B蛋白,其中E2和NS5A蛋白携带的高亲和力抗原表位较为丰富。结论 NS5B蛋白所携带的MHC-Ⅱ类抗原表位兼顾了各个HCV基因型之间的保守性及与MHC-Ⅱ类分子的高亲和力,具有较好的疫苗应用前景;NS3、E2、NS5A和NS4A蛋白有可能成为HCV多表位肽疫苗的备选组分。

Objective To predict the epitopes of hepatitis C virus ( HCV ) of different genotypes , which can be recognized by MHC-Ⅱmolecules , and provide a theoretical basis for HCV vaccine development. Methods Ten complete amino acid sequences of HCV were searched from NCBI database , which contained six geno-types of HCV.Amino acids sequence were analysed by program NetMHCIIpan-2.1 Server on line.The focuses are on the conservation of MHC-Ⅱepitopes among different HCV genetypes and affinity between the MHC-Ⅱ epitopes and MHC-Ⅱmolecules. Results NS3and NS5Bproteins contained the MHC-Ⅱ epitopes which were more conserved , and E2 , NS5A , NS4A and NS5B proteins contained the MHC-Ⅱ epitopes which had more affinity with the MHC-Ⅱmolecules. Conclusion NS5B may be an excellent MHC-Ⅱepitopes which is more conserved and have more affinity with the MHC-Ⅱmolecules.Also NS3 , E2 , NS5A and NS4A protein may be used as the alternative constitution for the multi-epitope peptide vaccine.