摘要
通过对六种大孔树脂的筛选,选出SP-700作为最佳的分离纯化多酚的大孔树脂,并且确定分离的基本条件是:2 mg/mL的粗多酚,pH为2,上样的流速为2.1 mL/min;洗脱剂为90%的乙醇,解吸液的流速为0.8 mL/min,纯化以后的多酚含量由一开始的17%增加到后来的71%。
Through screening six macroporous resin,SP-700 was selected as the best macroporous resin.And the optimum absorption conditions are concentration of polyphenol 2 mg/mL,pH 2,flowing rate 2.1 mL/min.90% ethanol is the eluteethanol,elution rate 0.8 mL/min,with the polyphenols content increasing from 17% to 71%.
基金
山东省科技国际合作项目(2012GHZ22103)
