摘要
Objective To establish a simple and rapid method for the determination of 1,3-dideoxygalactonojirimycin in Bombycis Faeces,a potent glucosidase inihibitor,by HPLC.Methods A RP-HPLC method with fluorescence detection has been developed.Results The HPLC method developed in this research has a good reliability including accuracy and precision.The detection limit was less than 72 ng.Conclusion This method is sufficiently sensitive for determining 1,3-dideoxygalactonojirimycin in Bombycis Faeces and other related products.
Objective To establish a simple and rapid method for the determination of 1,3-dideoxygalactonojirimycin in Bombycis Faeces,a potent glucosidase inihibitor,by HPLC.Methods A RP-HPLC method with fluorescence detection has been developed.Results The HPLC method developed in this research has a good reliability including accuracy and precision.The detection limit was less than 72 ng.Conclusion This method is sufficiently sensitive for determining 1,3-dideoxygalactonojirimycin in Bombycis Faeces and other related products.
