摘要
Objective To establish a RP-HPLC method investigate the processing technique and mechanism of Eucommiae Cortex.Methods The RP-HPLC method was applied to simultaneously determining six ingredients,geniposidic acid,geniposide,genipin,chlorogenic acid,(+)-pinoresinol-di-β-D-glucopyranoside,and(+)-syringaresinol-di-β-D-glucopyranoside,in the different processed barks of Eucommia ulmoides.Results The valid method with good accuracy could be well used to study the processing technique of E.ulmoides;Besides,target ingredients in E.ulmoide were decreased within 6 h when they were processed.Conclusion Established RP-HPLC is a reliable method which could be used to research the processing technique of the barks of E.ulmoides.Moreover,the result of this study could be provided with significant evidence of processed barks of E.ulmoides.
Objective To establish a RP-HPLC method investigate the processing technique and mechanism of Eucommiae Cortex.Methods The RP-HPLC method was applied to simultaneously determining six ingredients,geniposidic acid,geniposide,genipin,chlorogenic acid,(+)-pinoresinol-di-β-D-glucopyranoside,and(+)-syringaresinol-di-β-D-glucopyranoside,in the different processed barks of Eucommia ulmoides.Results The valid method with good accuracy could be well used to study the processing technique of E.ulmoides;Besides,target ingredients in E.ulmoide were decreased within 6 h when they were processed.Conclusion Established RP-HPLC is a reliable method which could be used to research the processing technique of the barks of E.ulmoides.Moreover,the result of this study could be provided with significant evidence of processed barks of E.ulmoides.
作者
WANG Jia-long1,LIU Er-wei1,2,WU Shuai1,YIN Zhao-ye3,ZHANG Yi2 1.Institute of Traditional Chinese Medicine Research,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China 2.Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China 3.School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072,China
基金
International Traditional Chinese Medicine Program for Corporation in Science and Technology (2008DFB30070)
Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT)
Tianjin Science and Technology Plan Project (10ZCKFSY09100)
