99例新生儿ABO溶血病血清学实验结果分析

The analysis of 99 cases of neonatal ABO hemolytic disease serological test

目的通过分析新生儿ABO溶血病(ABO-HDN)的高胆红素血症及血清学检测结果,系统性回顾评价血清学检测诊断方法,探讨ABO-HDN的血清学检查结果与高胆红素血症的关系。方法对某院99例确诊为ABO-HDN进行相关血清学实验室检测及同时段的血清胆红素检测。结果 99例ABO-HDN中热抗体放散实验阳性率达到97.98%,游离抗体实验阳性率为76.74%,直接抗人球蛋白实验阳性率为35.35%;出生后30 min内就检标本中高胆红素血症0例,发病率为0%,随着就检时间的延长,ABO-HDN发病率越高,高胆红素血症的阳性率也越大,并且A组的ABO-HDN阳性率(高胆红素血症阳性率)与B-I组有明显差异,具有统计学意义(P<0.05)。结论临床上应该在出现病理性黄疸之前进行新生儿溶血病3项血清学检查,热抗体释放试验是诊断新生儿溶血病最敏感的指标,并且在出生后30 min内进行血清学检查的临床意义最大。

Objective To investigate the relationship of ABO HDN serologic test results to high blood bilirubin,through the analysis of high blood bilirubin of ABO hemolytic disease of newborn(ABO HDN) and serological test results,as well as a systemic review evaluation serological detection diagnosis method.Methods The related serological tests and serum bilirubin from 99 cases diagnosed with ABO HDN were detected at the same time.Results Results showed that 99 cases of ABO hemolytic disease of newborn hot antibodies in children with radiation experiments positive rate reached up to 97.98%,free antibody test positive rate was 76.74%;by contrast the direct anti globulin test positive accounted for only 35.35%;Specimens of 30 minutes after birth to check high bilirubin levels,0 cases,the incidence was 0%.With the prolonging of the test time,the higher incidence of newborn children ABO hemolytic disease,the higher blood bilirubin positive rate.Moreover,the high bilirubin positive rate of ABO HDN group A compared with group B ～ I were significant differences statistically(P < 0.05).Conclusion Three serologic test for neonatal hemolysis disease should be carried out before pathologic jaundice was observed in the clinical.Hot antibody release test was the most sensitive indicators for diagnosis of hemolytic disease of newborn,and serological examination within half an hour after birth is the most significantly in the clinical.