不同模式林业血防工程对钉螺AST、ALT、ALP和SDH的影响

Impact on activity of AST,ALT,ALP and SDH for snails in project of afforestation for schistosomiasis control with different modes

目的阐明不同模式林业血防工程对钉螺AST、ALT、ALP、SDH酶活性的影响。方法分别选择全垦间种、全垦不种及不垦不种3种不同模式林业血防工程环境,同时在邻近区域各选择1块未实施林业血防工程的草滩或芦苇滩环境,采集各不同环境钉螺,分别测定各环境钉螺AST、ALT、ALP、SDH酶活性。结果林地环境钉螺谷丙转氨酶(AST)、谷草转氨酶(ALT)、碱性磷酸酶(ALP)活性均明显高于草滩或芦苇滩环境钉螺,琥珀酸脱氢酶(SDH)活性均低于草滩或芦苇滩环境。不同模式林业血防工程,钉螺AST、ALT、ALP、SDH活性水平均以全垦间种环境最高、其次为全垦不种,不垦不种最低。结论林业血防工程生态环境的改变,对钉螺体内酶组织化学产生一定的影响,干扰了钉螺体内的能量代谢,从而影响钉螺的生存繁殖。全垦间种林业血防工程模式对钉螺体内酶活性水平影响最大,其控制钉螺的效果将更明显。

Objective To clarify the influence on activity of AST,ALT,ALP and SDH for snails in project of afforestation for schistosomiasis control with different modes.Methods Three spots with different modes,cultivation with interval plantation,cultivation without plantation,no cultivation and plantation were selected in the project of afforestation for schistosomiasis control in Anhui Province,and three spots with grass land or reeds nearby were also selected.Snails in the spots selected were collected to assay the activity of AST,ALT,ALP and SDH.Results The activity of AST,ALT,and ALP for snails in woodland was obviously higher than that in grass land or reeds land,and SDH for snails was lower in woodland.The activity of AST、ALT、ALP and SDH for snails all was the highest in the mode of cultivation with interval plantation,the next was in the mode of cultivation without plantation,and the lowest was in the mode of no cultivation and plantation.Conclusion The change of ecological environment caused by project of afforestation for schistosomiasis control had certain influence on enzyme histochemistry of snails,which world disturb energetic metabolism of snails and affect the survival and reproduction on snails.The effect on snail control in the mode of cultivation with interval plantation would be better for its obviously influence on enzymatic activity of snails.